Agro-Industrial Wastes: A Substrate for Multi-Enzymes Production by Cryphonectria parasitica

This study aims to produce a mix of enzymes through Solid State Fermentation (SSF) raw materials. Four different, easily available, agro-industrial wastes were evaluated as SSF substrates for production by Cryphonectria parasitica (Murr.) Barr. environmental strains named CpA, CpB2, CpC4, and CpC7. Among the tested wastes, organic wheat bran human use animal feed better supports C. growth protease without any supplements. SDS-PAGE analyses highlighted presence three bands corresponding an extracellular laccase (77 kDa), endothiapepsin (37 carboxylesterase (60.6 kDa). Protease, laccase, esterase activities in 15 days, showing maximum activity at day 9 (3955.6 AU/gsf,). Conversely, best was achieved after days. The hypovirulent CpC4 strain showed highest (93.8 AU/gsf 2.5 U/gsf, respectively). These results suggest feasibility large-scale industrially relevant process on low value